Clontech 质粒pegfp-n1

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August undefined, 2024

Web质粒是一种独立于染色体外独立复制的双链DNA片段，通常都含有基因。. 天然存在的野生型质粒分子量大 (＞15kb)、拷贝数低、单一酶切位点少、遗传标记不理想等缺陷，不能满足克隆载体的要求。. 因此，天然质粒必须经过改造才能成为科研工作者的工具：. 1. 减 ... Web18 rows · Name Vector Type Resistance Marker Bacterial Resistance Source Sequence …

EcoO109 I pEGFP-C1 - Addgene

WebMay 3, 2006 · Clontech Laboratories, Inc. A Takara Bio Company 1290 Terra Bella Ave. Mountain View, CA 94043 Technical Support (US) E-mail: [email protected] www.clontech.com Vector Information pAcGFP1-N1 Vector Information PT3716-5 Cat. Nos. 632469 632426. Restriction Map and Multiple Cloning Site (MCS) of pAcGFP1-N1 … WebpEGFP-N1 (CLONTECH Lab., Palo Alto, CA, USA) has been modified to the dual-cassette expression vectors named pEGFP-Ks by the relocation of its ... MCS in the pEGFP-N1. The pEGFP-K2 vector contains ... my life clothes at walmart

of pEGFP-N1 Vector. - PKCLab.org - YUMPU

http://www.qualityard.com/upload/2024-05-24/a7a6fbe589a15b5dd4f2c88c144c656d.pdf WebpEGFP-N1 Vector Information PT3027-5 GenBank Accession #U55762 Catalog #6085-1 … http://www.synthesisgene.com/vector/pEGFP-N3.pdf mylife clydebank

pEGFP-N1TNFR 1 载体的构建及鉴定 - 豆丁网

WebMar 19, 1999 · pEGFP-N1 Vector Information PT3027-5 GenBank Accession #U55762 Catalog #6085-1 Restriction Map and Multiple Cloning Site (MCS) of pEGFP-N1 Vector. (Unique restriction sites are in bold.) Web拿大家比较熟悉的pegfp-c1和pegfp-n1做例子。想用n1质粒，设计引物就得把下游引物上的中止密码子去掉，不要辛辛苦苦的一路做下来结果根本不表达融合蛋白，你就死了;c1质粒，注意frame，要是移码了，你也死了。而且pegfp系列有1.2.3，要弄清楚别弄窜了。 my life clotheshttp://www.synthesisgene.com/vector/pEGFP-C1.pdf my life coach ds

"WebOct 3, 2002 · Restriction Map and Multiple Cloning Site (MCS) of pEGFP-C1. All restriction sites shown are unique. The Xba I and Bcl I sites (*) are methylated in the DNA provided by BD Biosciences Clontech. If you wish to digest the vector with these enzymes, you will need to transform the vector into a dam– host and make fresh DNA. Description " - Clontech 质粒pegfp-n1

Clontech 质粒pegfp-n1

WebThermo Scientific 分子克隆. 灵活。. 创新。. 快速。. Thermo Scientific CloneJET PCR 克 … WebApr 7, 2005 · 多克隆位点区. Restriction Map and Multiple Cloning Site of pEGFP-N1. (Unique restriction sites are in color or bold.) The Not I site follows the EGFP stop codon. The Xba I site (*) is methylated in the DNA provided by CLONTECH. If you wish to digest the vector with this enzyme, you will need to transform the vector into a dam-host and …

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http://www.bioon.com.cn/protocol/showarticle.asp?newsid=2628&typename=DNA%E7%80%B9%E7%82%BA%E7%8D%99&sortid=1737&typeid=2413 WebMay 3, 2006 · Clontech Laboratories, Inc. A Takara Bio Company 1290 Terra Bella Ave. …

WebJun 6, 2015 · required, stable transformants can be selected using G418 (7). pEGFP - N1 can also be used simply to express EGFP. in a cell line of interest (e.g., as a transfection marker). Location of features: • Human cytomegalovirus (CMV) immediate early promoter: 1–589. Enhancer region:59–465; TATA box: 554–560. WebpEGFP-N1,pEGFP N1载体价格1000元，pEGFPN1载体质粒图谱(Vector map),载体序 …

Web丁香通为您提供荧光蛋白报告载体质粒Cl商品详情介绍：价格：￥200 - 900，货 … Web1. Centrifuge at 5,000×g for 5 min. 2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

WebpEGFP-N1 Vector Information PT3027-5 GenBank Accession #U55762 Catalog #6085-1 Restriction Map and Multiple Cloning Site (MCS) of pEGFP-N1 Vector. ... pEGFP-N1 Vector Information BD Biosciences Clontech www.bdbiosciences.com Protocol # PT3027-5 2 Version # PR29972 Location of features • Human cytomegalovirus (CMV) immediate …

WebpEGFP-N1和pEGFP-N1-EMP1质粒转染Tb3.1细胞48 h后 GFP的表达荧光显微镜 × 100. Fig 3 GFP expression in the Tb3.1 cells transfected with pEGFP-N1 and pEGFP-N1-EMP1 after 48 hours fluorescence microscope × 100. 上：pEGFP-N1质粒；下：pEGFP-N1-EMP1重组质粒；左：绿色荧光；右：明场。 my life c murderWeb哺乳表达质粒 pEGFP-C1. 哺乳表达质粒 pEGFP-N1. 哺乳表达质粒 pEGFP-MHF. 哺乳表 … my life coffee alexanderWebSnapGene Viewer is free software that allows molecular biologists to create, browse, and share richly annotated sequence files. Gain unparalleled visibility of your plasmids, DNA and protein sequences. Annotate features on your plasmids using the curated feature database. Store, search, and share your sequences, files and maps. my life coffee lê duẩnWebClontech: Analyze: Sequence: Plasmid Type: Mammalian Expression: Promotor: CMV: … mylife coffee alexandre de rhodesWebpEGFP-N1 encodes a red-shifted variant of wild-type GFP which has been optimized for brighter fluorescence and higher expression in mammalian cells. (Excitation maximum = 488 nm; emission maximum = 507 nm.) pEGFP-N1 encodes the GFPmut1 variant which contains the double-amino-acid substitution of Phe-64 to Leu and Ser-65 to Thr. The … mylife coffeeWebE-mail: [email protected] www.clontech.com Vector Information pEGFP-N3 Vector InformationGenBank Accession #: U57609 Catalog #6080-1 PT3054-5 (PR29967; published 03 October 2002) Description: pEGFP-N3 encodes a red-shifted variant of wild-type GFP (1–3) which has been optimized for brighter fluorescence and higher expression in … mylife.com and marsha steinbergWebSep 8, 2016 · 本实验设计计划通过基因工程的方法，利用大肠杆菌表达系统表达增强型绿色荧光蛋白，首先pEGFP-N3和pET-28a (+)质粒从含有相应质粒的的E.coli中提取出来，然后利用双酶切法将两种质粒切开再利用T4连接酶构建pET-28a-EGFP质粒DNA，将重组质粒转入E.coliDH5α中，最后 ... mylife.com andries welding