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WebThe system is unique and flexible due to its dependence on RNA as the element that targets the nuclease to a desired DNA sequence. It can be used to induce indel mutations, specific sequence replacements/insertions, and large deletions or genomic rearrangements at any desired location in the genome.WebMay 24, 2024 · The chip was washed with TE buffer for 3 times, followed by blocking with 200 mg/mL BSA in PBS buffer and incubated with IRDye800 (Licor Biosciences) or Cy5 (GLPBIO) labeled streptavidin in PBS buffer with 200 mg/mL BSA, the incubation lasted for 30 min at room temperature in dark.great pacific tours san francisco
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